This PT sample is designed for the quantitative analysis of Legionella in water.

The PT sample is provided in duplicate as a stable dehydrated vitroid requiring hydration. The additional sample is provided for mishaps in the lab.

A 100 mL of sterile hydration fluid is supplied with the PT sample. This is a phosphate buffer.

Store the PT sample at 4°C.

The dehydrated vitroid sample is stable for a time exceeding the study schedule.

Due to the sensitivity of microorganisms and short holding times after hydration, please read entire instructions prior to preparation.

This sample is a single vitroid and the possible concentration range is quite broad, it may be necessary to analyze the sample in a dilution series using general phosphate buffer solution.

The hydration temperature of 35-37°C is rigorous. Low temperature will not assure complete hydration. Higher temperature will kill the microorganisms.

Allow the capped sample vial to equilibrate to room temperature.

While the sample is equilibrating to room temperature, warm the hydrating fluid in your incubator to 35-37°C. This will take about 60 minutes.

After the hydrating fluid is warmed to 35-37°C, open the sample vial and aseptically transfer the vitroid to the hydrating fluid. NOTE: The vitroid is the pink disk on top of the cotton. The beads below the cotton keep container dry and are not biologicals.

Shake well and place the sample in your incubator for 15 minutes.

The hydration temperature of 35-37°C is rigorous. Low temperature will not assure complete hydration. Higher temperature will kill the microorganisms.

After 15 minutes, remove the hydrated sample from the incubator and shake well. Inspect to assure dissolution of the vitroid. The disk will dissolve completely.

This represents the sample for analysis. Further dilutions are recommended to achieve a proper count, but base all counts on the whole volume sample.