This PT sample is designed for the quantitative analysis heterotrophic bacteria.

The PT sample is provided as a stable lyophilized pellet requiring hydration.

A 100 mL of sterile hydration fluid is supplied with the PT sample.

When prepared according to instructions, the concentration range of the sample will be between 5-500 CF/mL as per NELAC Fields of Testing, Jun 05.

The lyophilized sample is stable for a time exceeding the study schedule.

General Information:

Due to the sensitivity of microorganisms and short holding times after hydration, please read entire instructions prior to preparation. You must follow these instructions exactly to ensure viability of the lyophilized microorganisms.

This sample is a single pellet and the possible concentration range is quite broad, it may be necessary to analyze the sample in a dilution series.

This sample may be analyzed by any method appropriate for heterotrophic plate count.

Allow no more than 30 minutes to elapse from the completion of hydration and introduction of sample to media.

Preparation Instructions:

Allow the capped sample vial to equilibrate to room temperature. This may take up to an hour.

While the sample is equilibrating to room temperature, warm the hydrating fluid to your incubator to 35-37°C.

Once the sample is at room temperature and the hydrating fluid is warmed to 35-37°C (about 30 min), open the sample vial and aseptically transfer the pellet to the hydrating fluid. NOTE: The vitroid is the colored disk on top of the cotton. The beads below the cotton keep container dry and are not biologicals. Shake gently and place the sample in your incubator for 30 minutes.

The hydration temperature of 35-37°C is rigorous. Low temperature will not assure complete hydration. Higher temperature will kill the microorganisms.

After 30 minutes, remove the hydrated sample from the incubator and mix by inversion or vortexing. Inspect to assure dissolution of the pellet. If the pellet is not completely dissolved, return to bath for no more than 5 minutes and mix again. This represents the sample for analysis by your normal methods.

This PT sample is designed for the quantitative analysis heterotrophic bacteria.

The PT sample is provided as a stable lyophilized pellet requiring hydration.

A 100 mL of sterile hydration fluid is supplied with the PT sample.

When prepared according to instructions, the concentration range of the sample will be between 5-500 CF/mL as per NELAC Fields of Testing, Jun 05.

The lyophilized sample is stable for a time exceeding the study schedule.

General Information:

Due to the sensitivity of microorganisms and short holding times after hydration, please read entire instructions prior to preparation. You must follow these instructions exactly to ensure viability of the lyophilized microorganisms.

This sample is a single pellet and the possible concentration range is quite broad, it may be necessary to analyze the sample in a dilution series.

This sample may be analyzed by any method appropriate for heterotrophic plate count.

Allow no more than 30 minutes to elapse from the completion of hydration and introduction of sample to media.

Preparation Instructions:

Allow the capped sample vial to equilibrate to room temperature. This may take up to an hour.

While the sample is equilibrating to room temperature, warm the hydrating fluid to your incubator to 35-37°C.

Once the sample is at room temperature and the hydrating fluid is warmed to 35-37°C (about 30 min), open the sample vial and aseptically transfer the pellet to the hydrating fluid. NOTE: The vitroid is the colored disk on top of the cotton. The beads below the cotton keep container dry and are not biologicals. Shake gently and place the sample in your incubator for 30 minutes.

The hydration temperature of 35-37°C is rigorous. Low temperature will not assure complete hydration. Higher temperature will kill the microorganisms.

After 30 minutes, remove the hydrated sample from the incubator and mix by inversion or vortexing. Inspect to assure dissolution of the pellet. If the pellet is not completely dissolved, return to bath for no more than 5 minutes and mix again. This represents the sample for analysis by your normal methods.