PLEASE READ ALL OF THESE INSTRUCTIONS BEFORE BEGINNING ANALYSIS.

The method of analysis for this sample is USEPA Method 100.1/100.2, Determination of Asbestos Structures Over 10 µm in Length in Drinking Water.

In addition to the analytical requirements of Method 100.2, the laboratory must also perform the following approximately one hour before beginning analysis:

Vigorously shake the ampule for approximately 15 seconds.

Sonicate the ampule in an ultrasonic bath for at least 30 minutes.

Shake the ampule again for approximately 15 seconds.

Place the ampule in the ultrasonic bath again for another 30 minutes and continue to sonicate until it is opened.

Place 100 mL of freshly distilled water into a very clean (devoid of organic materials of  bacterial origin) 500 mL glass bottle with a screw cap.

Break the ampule at the pre-scored neck and transfer 1.0 mL of the material to the glass bottle containing the 100 mL distilled water, and dilute to 500 mL with freshly distilled water.

Shake the bottle vigorously for 15 seconds.

Adjust the pH of the suspension to between 3 and 4 with glacial acetic acid. [Typically, no more than a couple of milliliters is necessary. Add the acid with a micro-pipet or Pastuer pipet, and check the pH with non-bleed pH paper. After each addition of glacial acetic acid, shake the container vigorously before checking the pH.

Place the bottle in an ultrasonic bath for at least 30 minutes.

Shake the bottle vigorously before filtration.

Use a filtration apparatus with straight vertical sides to avoid losing fibers on tapered funnel sides.

Filter 50 mL of the sample.  Analyze per Method 100.2

Report results in MFL (Million Fibers per Liter)